Meanwhile, skeletal muscle is also a site where ectopic adipose tissue occurs ( 1). Skeletal muscle is vital for healthy life considering its function in movement and physical activity. Dynamic membrane proteome of adipogenic and myogenic precursors in skeletal muscle highlights EPHA2 may promote myogenic differentiation through ERK signaling. Collectively, our study provided an insight into the distinct MBP profile between myogenic and adipogenic precursors in skeletal muscle and served as a solid basis for supporting the role of MBPs in regulating differentiation.-Zhang, X., Wang, L., Qiu, K., Xu, D., Yin, J. Noteworthily, EPHA2 was required for myogenic differentiation, and it may promote myogenic differentiation through ERK signaling. Furthermore, key MBPs in regulating cell differentiation were also characterized, including ITGB3, ITGAV, ITPR3, and EPHA2. Functional enrichment analysis uncovered that myogenic and adipogenic precursors showed significant differences in cytoskeleton organization, syncytium formation, environmental information processing, and organismal systems. A total of 85 differentially expressed MBPs ( P < 0.05 and fold change ≥1.2 or ≤0.83) between 2 precursors were detected via isobaric tags for relative and absolute quantitation (iTRAQ) assay, including 67 up-regulated and 18 down-regulated in myogenic precursors. Adipogenic and myogenic precursors with divergent differentiation potential were isolated from the longissimus dorsi muscle of neonatal pigs by the preplate method. Given the vital role of membrane proteins (MBPs) in cell signal perception, membrane proteomics was conducted to delineate mechanisms regulating differentiation of adipogenic and myogenic precursors in skeletal muscle. NEW Feature: Species selection feature added to List Upload and Conversion Tool when using Official Gene Symbol.įalse discovery rate (FDR) has been changed from approximate FDR to adaptive FDR.ĭocumentation for Benjamini and FDR has been updated.The balance of myogenic and adipogenic differentiation is crucial for skeletal muscle homeostasis. Updated the DAVID header menu and home page. DAVID 6.8 can still be found on our development site.ĭAVID 2021 Update released on our development site with DAVID Knowledgebase v2021q4. The latest DAVID publication in Nucleic Acids Research is now available in PubMed.ĭAVID 2021 Update released on our production site with DAVID Knowledgebase v2021q4. Publications of the Laboratory of Human Retrovirology and Immunoinformatics, Frederick National Laboratory for Cancer Research LHRI has also studied the function of novel anti-viral cytokines (Interluekine-27 and Interferon Lambda-1) and the innate immune responses for developing novel immunotherapies.Ī A Post Doctoral Fellow position available to study the regulation of HIV infection and replication in primary cells (macrophages, T cells, and dendritic cells) in our Basic Research Section.įorum for DAVID users to ask questions, suggest new functions and help other users by answering their questions. The Laboratory of Human Retrovirology and Immunoinformatics (LHRI: former LHR), Applied/ Developmental Research Directorate () has investigated drug resistance in people living with HIV/AIDS (PLWHA) in the National Institute of Allergy and Infectious Diseases (NIAID) clinical therapy since 2001 to define salvage or de novo therapies.
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